An eco-friendly novel approach for bioconversion of Saccharum spontaneum biomass to biofuel-ethanol under consolidated bioprocess

Quest for renewable/eco-friendly energy sources has received immense focus in recent years. Current study involved consolidated bioprocessing of Saccharum spontaneum biomass (SSB) for biofuel-ethanol generation in a 'one pot consolidated bioprocess' (OPCB). SSB was pretreated with protic ionic liquid, triethylamine-bisulfate ([TEA][HSO4]), saccharified in-situ with cellulase/xylanase enzymes, and the released sugars were fermented to ethanol. Pretreatment and saccharification processes were optimized under OPCB to achieve 2.70-fold increased sugar yield i.e. from 196.56 to 531.00 mg/g biomass. Fermentation of sugars yielded ethanol at 209.6 mg/g biomass at a bioconversion efficiency of 72.56 %. The pretreated SSB was comprehensively examined by/for XRD, NMR, SEM, FT-IR, and properties such as water retention capacity, surface area and cellulase adsorption ability to elucidate functional mechanisms of [TEA][HSO4] pretreatment.

Automated summary

In this study, a consolidated bioprocessing method was used to convert Saccharum spontaneum biomass into biofuel-ethanol. Through pretreatment and saccharification processes, the sugar yield was significantly increased and the efficiency of ethanol production was relatively high. Furthermore, the pretreated SSB was comprehensively examined to elucidate the functional mechanisms of the pretreatment.