期刊
BIOMATERIALS
卷 287, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2022.121646
关键词
-
资金
- Max Planck Society
This study demonstrates that changes in fibronectin presentation on the surface have a comprehensive effect on cell mechanosensing, independent of bulk mechanics. Reduction of surface hydrophilicity alters fibronectin adsorption strength, allowing cells to remodel fibronectin and migrate.
The established link between deregulated tissue mechanics and various pathological states calls for the elucidation of the processes through which cells interrogate and interpret the mechanical properties of their microenvironment. In this work, we demonstrate that changes in the presentation of the extracellular matrix protein fibronectin on the surface of viscoelastic silicone elastomers have an overarching effect on cell mechanosensing, that is independent of bulk mechanics. Reduction of surface hydrophilicity resulted in altered fibronectin adsorption strength as monitored using atomic force microscopy imaging and pulling experiments. Conse-quently, primary human fibroblasts were able to remodel the fibronectin coating, adopt a polarized phenotype and migrate directionally even on soft elastomers, that otherwise were not able to resist the applied traction forces. The findings presented here provide valuable insight on how cellular forces are regulated by ligand presentation and used by cells to probe their mechanical environment, and have implications on biomaterial design for cell guidance.
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