期刊
BIOMATERIALS
卷 287, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2022.121653
关键词
Pancreatic tumor-stroma; In vitro models; Organoids; 3D bioprinting; Cancer-on-a-chip
资金
- FCT/MEC (PIDDAC) [UIDB/50011/2020, UIDP/50011/2020, LA/P/0006/2020]
- Programa Operacional Competitividade e Internacionaliza?a?o (POCI) , in the component FEDER [PTDC/BTM-SAL/30503/2017]
- Programa Operacional Competitividade e Internacionalizacao (POCI)
- national funds through FCT/MCTES [CEEC/1048/2019]
- Portuguese Foundation for Science and Technology (FCT) [PTDC/BTM-SAL/30503/2017, DFA/BD/7692/2020, SFRH/BD/141718/2018]
- Fundação para a Ciência e a Tecnologia [PTDC/BTM-SAL/30503/2017, SFRH/BD/141718/2018] Funding Source: FCT
This article presents the latest advances in bioengineering methods for in vitro modeling of the biological features and microenvironment of pancreatic cancer stroma, as well as providing design guidelines. The overview provides valuable examples and starting guidelines for researchers interested in engineering and characterizing stroma-rich biomimetic tumor models.
Pancreatic cancer exhibits a unique bioarchitecture and desmoplastic cancer-stoma interplay that governs disease progression, multi-resistance, and metastasis. Emulating the biological features and microenvironment heterogeneity of pancreatic cancer stroma in vitro is remarkably complex, yet highly desirable for advancing the discovery of innovative therapeutics. Diverse bioengineering approaches exploiting patient-derived organoids, cancer-on-a-chip platforms, and 3D bioprinted living constructs have been rapidly emerging in an endeavor to seamlessly recapitulate major tumor-stroma biodynamic interactions in a preclinical setting. Gathering on this, herein we showcase and discuss the most recent advances in bio-assembling pancreatic tumor-stroma models that mimic key disease hallmarks and its desmoplastic biosignature. A reverse engineering perspective of pancreatic tumor-stroma key elementary units is also provided and complemented by a detailed description of biodesign guidelines that are to be considered for improving 3D models physiomimetic features. This overview provides valuable examples and starting guidelines for researchers envisioning to engineer and characterize stroma-rich biomimetic tumor models. All in all, leveraging advanced bioengineering tools for capturing stromal heterogeneity and dynamics, opens new avenues toward generating more predictive and patient-personalized organotypic 3D in vitro platforms for screening transformative therapeutics targeting the tumor-stroma interplay.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据