4.4 Article

One-Step Homogeneous Immunoassay for the Detection of Influenza Virus Using Switching Peptide and Graphene Quencher

期刊

BIOCHIP JOURNAL
卷 16, 期 3, 页码 334-341

出版社

KOREAN BIOCHIP SOCIETY-KBCS
DOI: 10.1007/s13206-022-00076-x

关键词

One-step homogeneous immunoassay; Switching peptide; Influenza-A and influenza-B virus; Lateral flow immunoassay

资金

  1. Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) - Ministry of Health & Welfare, Republic of Korea [HI19C1344]

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A one-step homogeneous immunoassay using the switching peptide H2 was developed for rapid detection of influenza viruses A and B. This method eliminates the need for washing steps and can be conducted in solution, with graphene used as a fluorescence quencher to enhance sensitivity.
One-step homogeneous immunoassay was developed for detecting influenza viruses A and B (Inf-A and Inf-B) using the switching peptide H2. As the fluorescence-labeled switching peptide dissociated from the binding pocket of detection antibodies, the fluorescence signal could be directly generated by the binding of Inf-A and Inf-B without washing (i.e., one-step immunoassay). For the one-step homogeneous immunoassay with detection antibodies in solution, graphene was labeled with the antibodies as a fluorescence quencher. To test the feasibility of the homogeneous one-step immunoassay, the stability of the antibody complex with the switching peptide was evaluated under different pH and salt conditions. The one-step homogeneous immunoassay with switching peptide was conducted using influenza virus antigens in phosphate-buffered saline and real samples with inactivated Inf-A and Inf-B spiked in serum. Finally, the one-step homogeneous immunoassay results were compared with those of commercially available lateral flow immunoassays.

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