期刊
BIOCHEMICAL ENGINEERING JOURNAL
卷 185, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.bej.2022.108538
关键词
Halophilic bacteria; Salinivibrio; PHB; PHBV
资金
- National Key Research and Develop- ment Program of China [2018YFA0900200]
- National Natural Sci- ence Foundation of China [31870075]
Strains of Salinivibrio genus can grow under wide range of NaCl concentration. Salinivibrio sp. TGB11 is a novel bacterium that can produce polyhydroxyalkanoates from sugars and volatile fatty acids. It has shown high production yield of poly(3-hydroxybutyrate) (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) under different culture conditions.
Strains of Salinivibrio genus are able to grow under wide range of NaCl concentration. Salinivibrio sp. TGB11, a novel bacterium screened from saltworks, was found capable of producing polyhydroxyalkanoates from sugars and volatile fatty acids. In shake flask cultures, the use of glucose, sucrose, acetate, and butyrate achieved poly(3-hydroxybutyrate) (PHB) titers of 5.00, 3.23, 0.77, and 0.88 g/L, respectively. The addition of 0.5 g/L propionate with glucose yielded 4.38 g/L poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), with a 3-hydroxyvalerate monomer content of 8.57 mol%, yet propionate concentration higher than 1.5 g/L severely inhibited cell growth. In fed-batch bioreactor studies, PHB titer of 100.22 g/L and PHBV titer of 59.30 g/L were achieved. The PHB production yield was 0.42 g/g, reaching 88% of the theoretical value. Salinivibrio sp. TGB11 showed the highest PHB titer and yield in Salinivibrio genus and could be a promising host for industrial purposes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据