期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 613, 期 -, 页码 180-186出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2022.05.044
关键词
Gastric cancer; miR-4742-5p; Rab43; Invasion
资金
- National Research Founda-tion (NRF) - Korean government [2017R1C1B2003970, 2021R1A2C20 05853, 2021R1A4A1031856]
- National Research Foundation of Korea [2021R1A4A1031856, 2017R1C1B2003970] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
miR-4742-5p is a microRNA identified in gastric cancer that plays a role in cancer progression and metastasis. Its overexpression promotes the proliferation, migration, and invasion of gastric cancer cells and induces epithelial-mesenchymal transition. Rab43 is identified as a potential target gene, and its expression is associated with the activation of AKT and NF-kB.
miRNA (miR)-4742-5p is a recently identified microRNA regarding progression and metastasis in gastric cancer (GC). However, the biological function of this novel miRNA is largely unknown. We identified that the miR-4742-5p expression level was variably increased in GC cell lines. Suppression of miR-4742-5p using miR-inhibitor reduced the proliferation, migration, and invasion of GC cells with high miR-4742-5p expression, whereas overexpression of miR-4742-5p-mimic enhanced the aforementioned properties in GC cells with low miR-4742-5p expression. miR-4742-5p expression induced the decreases of Zo1 and E-cadherin expression as well as the increases of vimentin and N-cadherin expression, leading to epithelial-mesenchymal transition (EMT) of cancer cells. RNA sequencing results indicated Ras-related GTP-binding protein 43 (Rab43) as a potential target gene. We identified that the expression of Rab43 is associated with activation of AKT and nuclear factor-kappa B (NF-kB) which are key oncogenic pathways in cancer cells. Our results demonstrate a new component in GC progression, promising a potential therapeutic strategy.(c) 2022 Elsevier Inc. All rights reserved.
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