Establishing an economical and widely accessible donation after circulatory death animal abattoir model for lung research using ex vivo lung perfusion

Background Ex vivo lung perfusion (EVLP), is a platform that allows simultaneous testing and treatment of the lungs. However, use of EVLP is costly and requires access to lab animals and accompanying facilities. To increase the use of EVLP for research, we developed a method to perform EVLP using abattoir procured lungs. Furthermore, we were also able to significantly decrease costs. Methods Six pair of lungs were procured from abattoir sheep. The lungs were then flushed and stored in ice for 3 h. A low-flow (20% of cardiac output) approach, a tidal volume of 6 ml/kg bodyweight and total perfusion time of 3 h were chosen. Perfusion fluids and circuits were self-made. Lung biopsies, perfusate collection, respiratory values, circulatory pressures were recorded and hourly blood gas analyses were performed. Results Mean pO(2) remained stable from 60 min (49.3 +/- 7.1 kPa) to 180 min (51.5 kPa +/- 8.0), p = 0.66. Pulmonary artery pressure remained <= 15 mm Hg and the left atrial pressure remained between 3 and 5 mm Hg and peak respiratory pressures <= 20 cmH(2)O. Lactate dehydrogenase increased from start (96.3 +/- 56.4 U/L) to the end of perfusion (315.8 +/- 85.0 U/L), p < 0.05. No difference was observed in ATP between procurement and post-EVLP, 129.7 +/- 37.4 mu mol/g protein to 132.0 +/- 23.4 mu mol/g, p = 0.92. Conclusions Sheep lungs, acquired from an abattoir, can be ex vivo perfused under similar conditions as lab animal lungs with similar results regarding e.g., oxygenation and ATP restoration. Furthermore, costs can be significantly reduced by making use of this abattoir model. By increasing accessibility and lowering costs for experiments using lung perfusion, more results may be achieved in the field of lung diseases.

Automated summary

Sheep lungs acquired from an abattoir can be perfused ex vivo under similar conditions as lab animal lungs, with similar results in terms of oxygenation and ATP restoration. Furthermore, costs can be significantly reduced by using this abattoir model. By increasing accessibility and lowering costs for lung perfusion experiments, more results can be achieved in the field of lung diseases.