期刊
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
卷 42, 期 8, 页码 987-1004出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/ATVBAHA.122.317559
关键词
blood platelets; granules; megakaryocytes; phosphoinositides
资金
- Agence Nationale de la Recherche (ANR) [ANR-18-CE14-0003-01]
- Fondation pour la Recherche Medicale [EQU202203014742]
- ANR [ANR-18-CE14-0003-01]
This study demonstrates that PIKfyve and its lipid products PtdIns5P and PtdIns(3,5)P-2 play crucial roles in regulating granule integrity in both megakaryocytes and platelets. Inhibition of PIKfyve leads to impaired granule integrity, affecting platelet aggregation and thrombus formation.
Background: Secretory granules are key elements for platelet functions. Their biogenesis and integrity are regulated by fine-tuned mechanisms that need to be fully characterized. Here, we investigated the role of the phosphoinositide 5-kinase PIKfyve and its lipid products, PtdIns5P (phosphatidylinositol 5 monophosphate) and PtdIns(3,5)P-2 (phosphatidylinositol (3,5) bisphosphate) in granule homeostasis in megakaryocytes and platelets. Methods: For that, we invalidated PIKfyve by pharmacological inhibition or gene silencing in megakaryocytic cell models (human MEG-01 cell line, human imMKCLs, mouse primary megakaryocytes) and in human platelets. Results: We unveiled that PIKfyve expression and its lipid product levels increased with megakaryocytic maturation. In megakaryocytes, PtdIns5P and PtdIns(3,5)P-2 were found in alpha and dense granule membranes with higher levels in dense granules. Pharmacological inhibition or knock-down of PIKfyve in megakaryocytes decreased PtdIns5P and PtdIns(3,5)P-2 synthesis and induced a vacuolar phenotype with a loss of alpha and dense granule identity. Permeant PtdIns5P and PtdIns(3,5)P-2 and the cation channel TRPML (transient receptor potential mucolipin) 1 and TPC (two pore segment channel) 2 activation were able to accelerate alpha and dense granule integrity recovery following release of PIKfyve pharmacological inhibition. In platelets, PIKfyve inhibition specifically impaired the integrity of dense granules culminating in defects in their secretion, platelet aggregation, and thrombus formation. Conclusions: These data demonstrated that PIKfyve and its lipid products PtdIns5P and PtdIns(3,5)P-2 control granule integrity both in megakaryocytes and platelets.
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