期刊
AQUATIC TOXICOLOGY
卷 249, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.aquatox.2022.106236
关键词
Copper; Metabolomics; Acute exposure
资金
- Key Laboratory of Control of Quality and Safety for Aquatic Products, Ministry of Agriculture and Rural Affairs, P. R. China
- Qinghai Science and Technology Department Project [2019-NK-A2]
- Beijing Municipal Bureau of Agriculture and Rural Affairs [pxm2021 179303 000022]
This study aimed to evaluate the physiological responses of golden trout to acute copper exposure. The results showed that at a concentration of 60 µg/L, the fish exhibited gluconeogenesis, pentose-phosphate pathway activation, and alteration of fatty acid composition in response to copper exposure. At a concentration of 120 µg/L, the fish showed signs of metabolic stress.
Despite the broad knowledge of copper-induced stress and toxicity, data on the physiological responses to acute copper exposure and the correlation of those activities to a generalized stress response are still limited. The present study aimed to assess the physiological responses of golden trout to overcome copper stress at concentrations of 60 mu g/L and 120 mu g/L after 96 h, respectively. The activities of glucose-6-phosphate dehydrogenase (G6PD) phosphoenolpyruvate carboxykinase (PEPCK) and NADPH/NADP(+) ratio were significantly increased, and metabolites including glucose 6-phosphate, fructose 1-phosphate and fatty acids significantly accumulated in fish liver, indicating that gluconeogenesis, the pentose-phosphate pathway, as well as alteration of the membrane fatty acid composition were activated to serve as a defense mechanism against 60 mu g/L of copper after 96 h. After exposure to 120 mu g/L of copper for 96 h, the NAD(+) and ATP contents, the activities of enzymes in the glycolytic pathway (phosphofructokinase, PFK and pyruvate kinase, PK) and mitochondrial respiratory chain complex I decreased significantly in fish liver. In addition, carbohydrates and MDA accumulated in golden trout after 120 mu g/L copper treatment. These results indicated that 120 mu g/L of copper exposure may induce a metabolic stress in golden trout after 96 h. The multi-marker approach allows us to reach a greater understanding of the effects of copper on physiological responses of golden trout.
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