4.7 Article

A real-time quantitative PCR assay using hydrolysis probes for monitoring scuticociliate parasites in seawater

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Microbiology

Identifying Inorganic Turbidity in Water Samples as Potential Loss Factor During Nucleic Acid Extraction: Implications for Molecular Fecal Pollution Diagnostics and Source Tracking

Rita B. Linke et al.

Summary: This study focused on investigating matrix effects on water quality analysis results and found that in sites with high inorganic turbidity, DNA extractability could be significantly improved or made possible by using an adapted protocol. The study emphasizes the necessity of considering inorganic turbidity as a potential loss factor in DNA extraction from water matrices.

FRONTIERS IN MICROBIOLOGY (2021)

Review Medical Laboratory Technology

Efficiency Correction Is Required for Accurate Quantitative PCR Analysis and Reporting

Jan M. Ruijter et al.

Summary: Efficiency correction is essential for meaningful interpretation of qPCR results, considering the actual amplification efficiency. Absolute quantification with an undiluted calibrator is recommended for unbiased efficiency-corrected results.

CLINICAL CHEMISTRY (2021)

Article Plant Sciences

An optimized protocol for stepwise optimization of real-time RT-PCR analysis

Fangzhou Zhao et al.

Summary: Optimizing primer design and qPCR parameters are crucial for improving primer efficiency and data analysis accuracy.

HORTICULTURE RESEARCH (2021)

Article Ecology

Organic matter reduces the amount of detectable environmental DNA in freshwater

Kees Bochove et al.

ECOLOGY AND EVOLUTION (2020)

Review Biochemistry & Molecular Biology

Phylogenetic Utility of rRNA ITS2 Sequence-Structure under Functional Constraint

Wei Zhang et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020)

Article Biochemistry & Molecular Biology

Further analyses on the phylogeny of the subclass Scuticociliatia (Protozoa, Ciliophora) based on both nuclear and mitochondrial data

Tengteng Zhang et al.

MOLECULAR PHYLOGENETICS AND EVOLUTION (2019)

Review Biotechnology & Applied Microbiology

The Ultimate qPCR Experiment: Producing Publication Quality, Reproducible Data the First Time

Sean C. Taylor et al.

TRENDS IN BIOTECHNOLOGY (2019)

Article Environmental Sciences

Quantification of Protozoa and Viruses from Small Water Volumes

J. Alfredo Bonilla et al.

INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH (2015)

Article Marine & Freshwater Biology

A survey of ciliates at the long-term sampling station Helgoland Roads, North Sea

Jinpeng Yang et al.

HELGOLAND MARINE RESEARCH (2014)

Review Food Science & Technology

Guidelines for validation of qualitative real-time PCR methods

S. Broeders et al.

TRENDS IN FOOD SCIENCE & TECHNOLOGY (2014)

Article Biochemical Research Methods

A New Method for Quantitative Real-Time Polymerase Chain Reaction Data Analysis

Xiayu Rao et al.

JOURNAL OF COMPUTATIONAL BIOLOGY (2013)

Article Biochemical Research Methods

Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction

Jian Ye et al.

BMC BIOINFORMATICS (2012)

Article Medical Laboratory Technology

The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

Stephen A. Bustin et al.

CLINICAL CHEMISTRY (2009)

Article Medical Laboratory Technology

Checklist for Optimization and Validation of Real-Time PCR Assays

Marijke Raymaekers et al.

JOURNAL OF CLINICAL LABORATORY ANALYSIS (2009)

Article Fisheries

Antigenic and cross-protection studies on two turbot scuticociliate isolates

C. Piazzon et al.

FISH & SHELLFISH IMMUNOLOGY (2008)

Article Microbiology

Predicting the sensitivity and specificity of published real-time PCR assays

Gordon H Lemmon et al.

Annals of Clinical Microbiology and Antimicrobials (2008)

Article Fisheries

Histopathology of experimental scuticociliatosis in turbot Scophthalmus maximus

L. Puig et al.

DISEASES OF AQUATIC ORGANISMS (2007)