期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 106, 期 13-16, 页码 5249-5259出版社
SPRINGER
DOI: 10.1007/s00253-022-12053-3
关键词
Chloroplast targeting; Covalent conjugation; Extrinsic protein; Protein delivery; Wheat protoplast
资金
- Council for Scientific and Industrial Research (CSIR), Government of India
In this study, a novel peptide-based macromolecule delivery strategy targeting chloroplasts was successfully developed, offering advantages such as non-invasiveness, biocompatibility, low-toxicity, and target-specific delivery.
Reports on chloroplast-targeted protein delivery using cell-penetrating peptides are scarce. In this study, a novel peptide-based macromolecule delivery strategy targeting chloroplasts was successfully developed in wheat mesophyll protoplasts. A peptide derived from the signal sequence of the chloroplast-targeted protein of ferredoxin-thioredoxin reductase catalytic chain of Spinacia oleracea with UniProtKB Id-P41348 exhibits properties of cellular internalization. DNase I was efficiently delivered into the chloroplast using 10 mu M cTP with an efficiency of more than 90%. This cell-penetrating peptide-mediated approach offers various advantages over the existing chloroplast targeting methods, such as non-invasiveness, biocompatibility, low-toxicity, and target-specific delivery. The present study shows that peptide-based strategies hold tremendous potential in the field of chloroplast biotechnology.
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