期刊
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷 194, 期 11, 页码 5255-5273出版社
SPRINGER
DOI: 10.1007/s12010-022-03969-7
关键词
Steam explosion; Periodic peristalsis; Fed-batch feeding; Fed-batch enzyme; High solids enzymatic hydrolysis; Lignocellulose
资金
- National Key R&D Program of China [2019YFB1503800]
- Transformational Technologies for Clean Energy and Demonstration (Strategic Priority Research Program of the Chinese Academy of Sciences) [XDA 21060300]
This study successfully prepared high-concentration fermentable sugars using multiple intensification strategies in high-solid enzymatic hydrolysis. Steam explosion pretreatment enhanced the hydrophilicity of substrates and enzymatic accessibility, periodic peristalsis improved mass transfer efficiency and shortened liquefaction time, and fed-batch feeding and enzyme addition reduced enzyme loading.
High concentrations of fermentable sugars are a demand for economical bioethanol production. A single process strategy cannot comprehensively solve the limiting factors in high-solid enzymatic hydrolysis. The multiple intensification strategies in this study achieved the goal of preparing high-concentration fermentable sugars of corn stalk with high solid loading and low enzyme loading. First, steam explosion pretreatment enhanced the hydrophilicity of substrates and enzymatic accessibility. Second, periodic peristalsis was used to improve the mass transfer efficiency and short the liquefaction time. Additionally, fed-batch feeding and enzyme reduced the enzyme loading. Ultimately, the intensification strategies above showed that the highest fermentable sugar content was 313.8 g/L with a solids loading as much as 50% (w/w) and enzyme loading as low as 12.5 FPU/g DM. Thus, these multiple intensification strategies were promising in the high-solid enzymatic hydrolysis of steam-exploded lignocellulose.
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