期刊
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
卷 66, 期 8, 页码 -出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/aac.00710-22
关键词
C. parapsilosis; fluconazole resistance; Y132F; G458S; microsatellite genotyping
资金
- Fondo de Investigacion Sanitaria (FIS. Instituto de Salud Carlos III
- Plan Nacional de I+D+I) [PI18/01155, PI19/00074]
- European Regional Development Fund (FEDER) 'A way of making Europe'
- Scynexis, Inc., Jersey City, New Jersey, USA
- FIS [CPII20/00015, FI19/00021]
This study monitored antifungal resistance in Candida parapsilosis isolates from inpatients at hospitals in the Madrid metropolitan area over a three-year period. The findings reveal the spread of fluconazole-resistant genotypes of C. parapsilosis across multiple hospitals in Madrid, Spain.
We have been monitoring the antifungal resistance in Candida parapsilosis isolates collected from inpatients at Madrid metropolitan area hospitals for the last 3 years. The study aimed to elucidate the presence of fluconazole-resistant C. parapsilosis genotypes in Madrid. From January 2019 to December 2021, a total of 354 C. parapsilosis isolates (n = 346 patients) from blood (76.6%) or intraabdominal samples were collected and genotyped using species-specific microsatellite markers. Antifungal susceptibilities to amphotericin B, the triazoles, micafungin, anidulafungin, and ibrexafungerp were performed according to EUCAST E.Def 7.3.2; the ERG11 gene was sequenced in fluconazole-resistant isolates. A total of 13.6% (n = 48/354) isolates (one per patient) were found to be resistant to fluconazole and non-wild-type to voriconazole but fully susceptible to ibrexafungerp. Resistant isolates were mostly sourced from blood (n = 45/48, 93.8%) and were detected in five hospitals. Two hospitals accounted for a high proportion of resistant isolates (n = 41/48). Resistant isolates harbored either the Y132F ERG11p amino acid substitution (n = 43) or the G458S substitution (n = 5). Isolates harboring the Y132F substitution clustered into a clonal complex involving three genotypes (one genotype accounted for n = 39/43 isolates) that were found in four hospitals. Isolates harboring the G458S substitution clustered into another genotype found in a fifth hospital. C. parapsilosis genotypes demonstrating resistance to fluconazole have been spreading across hospitals in Madrid, Spain. Over the last 3 years, the frequency of isolation of such isolates and the number of hospitals affected is on the rise.
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