期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 61, 期 37, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202203706
关键词
COVID-19; Microfluidic Immunoassays; Multiplexing; Rolling Circle Amplification; Tunable Detection Range
资金
- National Natural Science Foundation of China [82072087, 31970893]
- Science and Technology Projects in Guangzhou [202206010087]
This article introduces a new method for serological assay in COVID-19 patients, which achieves high throughput and sensitivity by integrating color-size dual-encoded beads and rolling circle amplification technology. The method can quantitatively detect COVID-19 antibodies and also detect inflammatory biomarkers. In addition, the detection range of this method is tunable and can be used to simultaneously measure more COVID-19 serological molecules differing by orders of magnitude.
Serological assay for coronavirus 2019 (COVID-19) patients including asymptomatic cases can inform on disease progression and prognosis. A detection method taking into account multiplex, high sensitivity, and a wider detection range will help to identify and treat COVID-19. Here we integrated color-size dual-encoded beads and rolling circle amplification (RCA) into a bead-based fluorescence immunoassay implemented in a size sorting chip to achieve high-throughput and sensitive detection. We used the assay for quantifying COVID-19 antibodies against spike S1, nucleocapsid, the receptor binding domain antigens. It also detected inflammatory biomarkers including interleukin-6, interleukin-1 beta, procalcitonin, C-reactive protein whose concentrations range from pg mL(-1) to mu g mL(-1). Use of different size beads integrating with RCA results in a tunable detection range. The assay can be readily modified to simultaneously measure more COVID-19 serological molecules differing by orders of magnitude.
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