期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 61, 期 34, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202206122
关键词
Biosensors; Brain; Neuropeptide Release; Neuropeptide Transmission; Plasmonic Nanovesicles
资金
- National Science Foundation [1631910]
- National Institute of Neurological Disorders and Stroke of the National Institutes of Health (NIH) [RF1NS110499]
- National Institute of Mental Health of NIH [R01MH111499]
- Human Frontier Science Program (HFSP) [RGP0036/2020]
- Phospholipid Research Center (Heidelberg, Germany)
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [1631910] Funding Source: National Science Foundation
In this study, an integrated optical approach using Plasmonic nAnovesicles and cell-based neurotransmitter fluorescent engineered reporter (CNiFER), called PACE, was developed to investigate neuropeptide signaling in the mouse neocortex. The results show that neuropeptide transmission is reduced but synchronized within 130 μm and smaller and delayed at longer distances.
Neuropeptides are abundant signaling molecules in the central nervous system. Yet remarkably little is known about their spatiotemporal spread and biological activity. Here, we developed an integrated optical approach using Plasmonic nAnovesicles and cell-based neurotransmitter fluorescent engineered reporter (CNiFER), or PACE, to probe neuropeptide signaling in the mouse neocortex. Small volumes (fL to pL) of exogenously supplied somatostatin-14 (SST) can be rapidly released under near-infrared light stimulation from nanovesicles implanted in the brain and detected by SST2 CNiFERs with nM sensitivity. Our measurements reveal reduced but synchronized SST transmission within 130 mu m, and markedly smaller and delayed transmission at longer distances. These measurements enabled a quantitative estimation of the SST loss rate due to peptide degradation and binding. PACE offers a new tool for determining the spatiotemporal scales of neuropeptide volume transmission and signaling in the brain.
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