Luminescence Lifetime Imaging Based on Lanthanide Nanoparticles

Luminescence lifetime imaging has exhibited significant advantages over traditional optical imaging in terms of precise quantification detection because of the intrinsic stability of luminescence lifetimes. Recently, great emphasis has been put on the exploration of lanthanide-doped nanoparticles (LnNPs) with long-lived luminescence. The long luminescence lifetime of LnNPs not only makes it easier to filter out background signals during imaging, but also provides a wide range of tunable lifetime. Here, we introduce the luminescence mechanisms of LnNPs, the key strategies for luminescence lifetime modulation, data acquisition methods, and several cutting-edge applications in lifetime imaging systems. Then we describe several prospects to inspire efforts for improving technologies and extending applications of luminescence lifetime imaging.

Automated summary

Luminescence lifetime imaging has advantages over traditional optical imaging in terms of precise quantification detection, and the long luminescence lifetime of lanthanide-doped nanoparticles (LnNPs) has attracted great attention. LnNPs with long luminescence lifetime not only make it easier to filter out background signals during imaging, but also provide a wide range of tunable lifetime. This article introduces the luminescence mechanisms of LnNPs, strategies for luminescence lifetime modulation, data acquisition methods, and cutting-edge applications in lifetime imaging systems, as well as prospects for improving technologies and extending applications of luminescence lifetime imaging.