期刊
ANALYTICAL CHEMISTRY
卷 94, 期 30, 页码 10567-10572出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.2c02279
关键词
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资金
- University of Oklahoma
- Burroughs Wellcome Fund
- National Institutes of Health [R01GM116116]
- National Science Foundation [OCE-1634630]
- Research Council of the University of Oklahoma Norman Campus
This study revealed heterogeneity in the metabolic response of host cells to Trypanosoma cruzi infection, with uninfected cells also showing metabolic impacts. These findings provide novel insights into the pathogenesis of Chagas disease.
Cellular heterogeneity is generally overlooked in infectious diseases. In this study, we investigated host cell heterogeneity during infection with Trypanosoma cruzi (T. cruzi) parasites, causative agents of Chagas disease (CD). In chronic-stage CD, only a few host cells are infected with a large load of parasites and symptoms may appear at sites distal to parasite colonization. Furthermore, recent work has revealed T. cruzi heterogeneity with regard to replication rates and drug susceptibility. However, the role of cellular-level metabolic heterogeneity in these processes has yet to be assessed. To fill this knowledge gap, we developed a Single-probe SCMS (single-cell mass spectrometry) method compatible with biosafety protocols, to acquire metabolomics data from individual cells during T. cruzi infection. This study revealed heterogeneity in the metabolic response of the host cells to T. cruzi infection in vitro. Our results showed that parasite-infected cells possessed divergent metabolism compared to control cells. Strikingly, some uninfected cells adjacent to infected cells showed metabolic impacts as well. Specific metabolic changes include increases in glycerophospholipids with infection. These results provide novel insight into the pathogenesis of CD. Furthermore, they represent the first application of bioanalytical SCMS to the study of mammalian-infectious agents, with the potential for broad applications to study infectious diseases.
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