期刊
ANALYTICAL CHEMISTRY
卷 -, 期 -, 页码 -出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.2c01918
关键词
-
资金
- National Natural Science Foundation (NNSF) of China [21974108, 212176153, 212174113]
- Fundamental Research Funds for the Central Universities, China [SWU118106]
A novel region recognition method for Pre-miRNA based on HB-HCR amplification was constructed to eliminate interference and achieve simultaneous sensitive detection and imaging of Pre-miRNA and miRNA. This method is of great significance in the diagnosis of tumor diseases.
Herein, a novel region recognition of precursor microRNA (Pre-miRNA) based on hyperbranched hybrid chain reaction (HB-HCR) amplification was constructed to effectively eliminate the interference of Pre-miRNA to the mature microRNA (miRNA) by establishing the linear mapping relation between the two fluorescence signals produced by the miRNA sequence in the Pre-miRNA and Pre-miRNA residues to first realize simultaneous sensitive detection of Pre-miRNA and miRNA as well as highly sensitive imaging of intracellular Pre-miRNA and miRNA, which solves one main challenge of in vitro tumor disease diagnostics: inaccurate detection of tumor-induced miRNA changes. Impressively, this strategy easily distinguishes cancer cells from normal cells and DNA-damaged cells by the difference in miRNA and Pre-miRNA expression, which provides an innovative approach for accurate clinical diagnosis of cancer and precise treatment of prognosis.
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