期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 414, 期 24, 页码 7123-7141出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-022-04253-7
关键词
Exosome; Extracellular vesicle; Microfluidic; Isolation; Separation
资金
- Cuiying Scientific and Technological Innovation Program of Lanzhou University Second Hospital [CY2021-QN-A19]
- A joint project of TCM and western medicine to tackle major diseases, Robot-assisted precise treatment solution for scoliosis [KXW-2021-J]
- Cuiying Scientific Training Program for Undergraduates of Lanzhou University Second Hospital [CYXZ2020-02]
This review analyzes the progress in exosomal isolation strategies and proposes views on the development prospects of various exosomal isolation techniques.
Abstract Exosomes are extracellular vesicles with a diameter ranging from 30 to 150 nm, which are an important medium for intercellular communication and are closely related to the progression of certain diseases. Therefore, exosomes are considered promising biomarkers for the diagnosis of specific diseases, and thereby, treatments based on exosomes are being widely examined. For exosome-related research, a rapid, simple, high-purity, and recovery isolation method is the primary prerequisite for exosomal large-scale application in medical practice. Although there are no standardized methods for exosome separation and analysis, various techniques have been established to explore their biochemical and physicochemical properties. In this review, we analyzed the progress in exosomal isolation strategies and proposed our views on the development prospects of various exosomal isolation techniques.
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