期刊
ANALYTICA CHIMICA ACTA
卷 1221, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.aca.2022.340103
关键词
Metal-organic framework; Sulfur quantum dots; Fluorescence; Alkaline phosphatase; Ascorbic acid
资金
- National Natural Science Foundation of China [21804030, 22007027]
- Natural Science Interdisciplinary Research Program of Hebei University [DXK201906]
- Research Innovation Team of College of Chemistry and Environmental Science of Hebei University [hxkytd-py2101]
- Post-graduate's Innovation Fund Project of Hebei Province [CXZZBS2020019]
- Post-graduate's Innovation Fund Project of Hebei University [HBU2022ss031]
Sulfur quantum dots (SQDs) were successfully encapsulated into metal-organic frameworks (ZIF-8) using a surface bound zinc ion-induced growth strategy, resulting in SQDs@ZIF-8 for alkaline phosphatase (ALP) detection. This new synthesis procedure significantly improved the luminous efficiency of SQDs. The detection of ALP was achieved by pH-triggered fluorescence quenching of SQDs@ZIF-8, and an extended detection range was obtained by complexing free SQDs with Fe2+. SQDs@ZIF-8 exhibited excellent stability in water and showed potential for fluorescence and colorimetric detection of ALP in human serum.
Controllable encapsulation of sulfur quantum dots (SQDs) into metal-organic frameworks (ZIF-8) by a surface bound zinc ion-induced growth strategy, and SQDs@ZIF-8 was successfully prepared for alkaline phosphatase (ALP) detection. The new synthesis procedure involves first binding Zn2+ to the surface of SQDs to form SQDs/ Zn, and then via zinc ion-induced in situ ZIF-8 growth to obtain SQDs@ZIF-8, which greatly improved the luminous efficiency of SQDs. The specific process of detecting ALP using pH-triggered fluorescence quenching of SQDs@ZIF-8: firstly ALP hydrolyzes 2-phosphate -L-ascorbic acid trisodium salt (AAP) to ascorbic acid (AA), and then the leakage of SQDs in the SQDs@ZIF-8 leads to a decrease in fluorescence intensity based on the destruction of ZIF-8 skeleton by H+ released by AA. A linear relationship was obtained between the fluorescence intensity and the ALP concentration in the range of 0.15-50 U/L, and the detection limit was 0.044 U/L. Moreover, it was found that free SQDs can be complexed with Fe2+ to produce wine red complexes, and the obtained UV absorbance and ALP concentration have a linear relationship in the range of 10-200 U/L. The detection range of ALP is significantly broadened based on the combination of the above two detection methods. Furthermore, SQDs@ZIF-8 exhibited excellent stability in water and was successfully applied to the fluorescence and colorimetric detection of ALP in human serum.
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