期刊
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
卷 323, 期 3, 页码 L251-L265出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajplung.00485.2021
关键词
behavior; cigarette smoke exposure; COPD; inflammation; lung-brain axis
资金
- Top Sector Life Sciences & Health -Top Consortia for Knowledge and Innovation (LSH-TKI) -Lung Foundation Netherlands Public -Private Partnership (PPP) allowance [10.2.16.119]
- Chinese Scholarship Council [201706170055]
Brain-related comorbidities are commonly observed in chronic obstructive pulmonary disease (COPD) and are associated with disease progression and mortality. This study used a mouse model to investigate the impact of cigarette smoke and bacterial trigger exposure on brain and behavior. The findings suggest that cigarette smoke exposure leads to pulmonary inflammation and neuroinflammation, resulting in increased exploratory behavior and compromised blood-brain barrier integrity.
Brain-related comorbidities are frequently observed in chronic obstructive pulmonary disease (COPD) and are related to increased disease progression and mortality. To date, it is unclear which mechanisms are involved in the development of brainrelated problems in COPD. In this study, a cigarette smoke and lipopolysaccharide (LPS) exposure murine model was used to induce COPD-like features and assess the impact on brain and behavior. Mice were daily exposed to cigarette smoke for 72 days, except for days 42, 52, and 62, on which mice were intratracheally exposed to the bacterial trigger LPS. Emphysema and pulmonary inflammation as well as behavior and brain pathology were assessed. Cigarette smoke-exposed mice showed increased alveolar enlargement and numbers of macrophages and neutrophils in bronchoalveolar lavage. Cigarette smoke exposure resulted in lower body weight, which was accompanied by lower serum leptin levels, more time spent in the inner zone of the open field, and decreased claudin-5 and occludin protein expression levels in brain microvessels. Combined cigarette smoke and LPS exposure resulted in increased locomotion and elevated microglial activation in the hippocampus of the brain. These novel findings show that systemic inflammation observed after combined cigarette smoke and LPS exposure in this COPD model is associated with increased exploratory behavior. Findings suggest that neuroinflammation is present in the brain area involved in cognitive functioning and that blood-brain barrier integrity is compromised. These findings can contribute to our knowledge about possible processes involved in brain-related comorbidities in COPD, which is valuable for optimizing and developing therapy strategies.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据