期刊
ACS NANO
卷 16, 期 7, 页码 10811-10823出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.2c02898
关键词
extracellular vesicles; exosomes; mesenchymal stem cells; angiogenesis; spinal cord injury
类别
资金
- National KeyResearch and Development Project of Stem Cell and Transformation Research [2019YFA0112100, 2019YFA0112102]
- National Natural Science Foundationof China [81973252, 81803451]
- Ten-thousand TalentsProgram of Zhejiang Province [2018R52049]
- Ningbo keyscientific and technological project [2021Z028]
- Morphological Platform,Zhejiang University School of Medicine
This study found that exosomes derived from hypoxic preconditioned human umbilical vein endothelial cells can stimulate angiogenic MSCs, showing great potential in the treatment of traumatic spinal cord injury.
ABSTRACT: Revascularization treatment is a critical measure for tissue engineering therapies like spinal cord repair. As proven to regulate the lesion microenvironment through feedback to the microenvironment signals. The angiogenic capacities of MSCs have been reported to be facilitated by vein endothelial cells in the niche. As emerging evidence demonstrated the roles of exosomes in cell-cell and cell-microenvironment communications, to cope with the ischemia complication for treatment of traumatic spinal cord injury, the study extracts the microenvironment factors to stimulate angiogenic MSCs through using exosomes (EX) derived from hypoxic preconditioned (HPC) human umbilical vein endothelial cells (HUVEC). The HPC treatment with a hypoxia time segment of only 15 min efficiently enhanced the function of EX in facilitating MSCs angiogenesis activity. MSCs stimulated by HPC-EX showed significant tube formation within 2 h, and the in vivo transplantation of the stimulated MSCs elicited effective nerve tissue repair after rat spinal cord transection, which could be attributed to the pro-angiogenic and antiinflammatory impacts of the MSCs. Through the simulation of MSCs using HPC-tailored HUVEC exosomes, the results proposed an efficient angiogenic nerve tissue repair strategy for spinal cord injury treatment and could provide inspiration for therapies based on stem cells and exosomes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据