期刊
ACS NANO
卷 16, 期 6, 页码 9004-9018出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.2c00285
关键词
nanoheater-thermometer; photothermal dye; NIR light; local heating; cell engineering
类别
资金
- Japan Agency for Medical Research and Development (AMED) PRIME [JP18gm5810001]
- JST FOREST Program (Japan) [JPMJFR201E]
- JSPS KAKENHI [JP20H04702, JP19H02750]
- World Premier International Research Center Initiative (WPI) , MEXT
Thermal engineering at the microscale presents a significant challenge for basic biological research and biomaterials development. Researchers have developed a nanoparticle that can generate heat spots within live cells and measure the temperature increment using fluorescence thermometry. The results demonstrate the effectiveness of a single heat spot in altering cellular functions.
Thermal engineering at the microscale, such as the regulation and precise evaluation of the temperature within cellular environments, is a major challenge for basic biological research and biomaterials development. We engineered a polymeric nanoparticle having a fluorescent temperature sensory dye and a photothermal dye embedded in the polymer matrix, named nanoheater-thermometer (nanoHT). When nanoHT is illuminated with a near-infrared laser at 808 nm, a subcellular-sized heat spot is generated in a live cell. Fluorescence thermometry allows the temperature increment to be read out concurrently at individual heat spots. Within a few seconds of an increase in temperature by approximately 11.4 degrees C from the base temperature (37 degrees C), we observed the death of HeLa cells. The cell death was observed to be triggered from the exact local heat spot at the subcellular level under the fluorescence microscope. Furthermore, we demonstrate the application of nanoHT for the induction of muscle contraction in C2C12 myotubes by heat release. We successfully showed heat-induced contraction to occur in a limited area of a single myotube based on the alteration of protein-protein interactions related to the contraction event. These results demonstrate that even a single heat spot provided by a photothermal material can be extremely effective in altering cellular functions.
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