期刊
ACS CHEMICAL BIOLOGY
卷 17, 期 10, 页码 2769-2780出版社
AMER CHEMICAL SOC
DOI: 10.1021/acschembio.2c00324
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资金
- National Institute of General Medical Sciences [P41 GM108569]
- NIH [S10OD025194, RF1AG063903]
- Thermo Fisher Scientific
- Chemistry of Life Processes Predoctoral Training Grant at Northwestern University [T32GM105538]
- Army Research Office [W911NF-16-1-0372, W911NF-18-1-0200]
- David and Lucile Packard Foundation
- Camille Dreyfus TeacherScholar Program
The phosphorylation of triosephosphate isomerase (TPI) enhances its activity and facilitates substrate binding to the active site. Hetero-dimerization and subunit asymmetry are key features of TPI, and tetramerization plays a crucial role in enzymatic regulation. This study highlights the importance of studying endogenous proteoforms for understanding the functional role of post-translational modifications (PTMs).
Triosephosphate isomerase (TPI) performs the 5th step in glycolysis, operates near the limit of diffusion, and is involved in moonlighting functions. Its dimer was found singly phosphorylated at Ser20 (pSer20) in human cells, with this post-translational modification (PTM) showing context-dependent stoichiometry and loss under oxidative stress. We generated synthetic pSer20 proteoforms using cell-free protein synthesis that showed enhanced TPI activity by 4-fold relative to unmodified TPI. Molecular dynamics simulations show that the phosphorylation enables a channel to form that shuttles substrate into the active site. Refolding, kinetic, and crystallographic analyses of point mutants including S20E/G/Qindicate that hetero-dimerization and subunit asymmetry are key features of TPI. Moreover, characterization of an endogenous human TPI tetramer also implicates tetramerization in enzymatic regulation. S20 is highly conserved across eukaryotic TPI, yet most prokaryotes contain E/D at this site, suggesting that phosphorylation of human TPI evolved a new switch to optionally boost an already fast enzyme. Overall, complete characterization of TPI shows how endogenous proteoform discovery can prioritize functional versus bystander PTMs.
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