Phone Camera Nano-Biosensor Using Mighty Sensitive Transparent Reusable Upconversion Paper

Lycopene, a natural colorant and antioxidant with a huge growing market, is highly susceptible to photo/thermal degradation, which demands real-time sensors. Hence, here a transparent upconversion nanoparticles (UCNPs) strip having 30 mol % Yb, 0.1 mol % Tm, and beta-NaYF4 UCNPs, which shows an intense emission at 475 nm, has been developed. This strip has been found to be sensitive to lycopene with a detection limit as low as 10 nM using a smartphone camera, which is due to static quenching that is confirmed by the lifetime study. In comparison to previous paper strips, here the transparent strip has minimal scattering with maximum sensitivity in spite of not using any metal quenchers. An increase in strip hydrophobicity during the fabrication process complements the strip to selectively permeate and present an extraction-free substitute analysis for chromatography. Hydrophobicity endows the strip with the capability to reuse the strip with similar to 100% luminescence recovery.

Automated summary

A transparent upconversion nanoparticles strip has been developed for real-time monitoring of lycopene concentration. The strip shows a low detection limit and increased hydrophobicity during fabrication, making it a potential extraction-free substitute for chromatography. Additionally, the strip exhibits a high luminescence recovery rate.