期刊
ACS APPLIED MATERIALS & INTERFACES
卷 -, 期 -, 页码 -出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.2c00920
关键词
decellularization; bioprinting; 3D in vitro cancer model; breast tissue
资金
- European Union [712754]
- Spanish Ministry of Economy and Competitiveness [SEV-2014-0425, CEX2018-000789-S]
- Tecnologies Emergents program of the General Directorate for Research-Generalitat de Catalunya (FEDER Operational Program of Catalonia 2014-2020) [001-P-001646]
- Programme/Generalitat de Catalunya [2017SGR-359]
- European Regional Development Fund (FEDER)
- Spanish Ministry of Science, Innovation and Universities [RTI2018-096320-B-C21, MAT201568906-R]
- Spanish Ministry of Economy, Industry and Competitiveness [EUIN2017-89173]
- CERCA Program/Generalitat de Catalunya
- European CommissionEuronanomed3 nAngioderm Project [JTC2018-103, PCI2019-103648]
- Marie Curie Actions (MSCA) [712754] Funding Source: Marie Curie Actions (MSCA)
Breast tissue-derived matrices can serve as important biomaterials to replicate the complexity of the tumor extracellular matrix (ECM), with the addition of collagen type I improving the fidelity to breast tumors. These novel bioinks have great potential in bioprinting breast cancer models.
The tumor extracellular matrix (ECM) plays a vital role in tumor progression and drug resistance. Previous studies have shown that breast tissue-derived matrices could be an important biomaterial to recreate the complexity of the tumor ECM. We have developed a method for decellularizing and delipidating a porcine breast tissue (TDM) compatible with hydrogel formation. The addition of gelatin methacrylamide and alginate allows this TDM to be bioprinted by itself with good printability, shape fidelity, and cytocompatibility. Furthermore, this bioink has been tuned to more closely recreate the breast tumor by incorporating collagen type I (Col1). Breast cancer cells (BCCs) proliferate in both TDM bioinks forming cell clusters and spheroids. The addition of Col1 improves the printability of the bioink as well as increases BCC proliferation and reduces doxorubicin sensitivity due to a downregulation of HSP90. TDM bioinks also allow a precise three-dimensional printing of scaffolds containing BCCs and stromal cells and could be used to fabricate artificial tumors. Taken together, we have proven that these novel bioinks are good candidates for biofabricating breast cancer models.
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