期刊
JOURNAL OF CLINICAL NEUROSCIENCE
卷 28, 期 -, 页码 141-147出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.jocn.2015.10.045
关键词
Filament perforation model; Magnetic resonance imaging; Mice; Subarachnoid hemorrhage
资金
- Swiss Foundation for Grants in Biology and Medicine
- Japan Society for the Promotion of Science
- Grants-in-Aid for Scientific Research [16H04664, 15K14335, 15H01446, 26462170] Funding Source: KAKEN
The filament perforation model (FPM) in mice is becoming increasingly popular to elucidate the molecular pathogenesis of neuronal injury after subarachnoid hemorrhage (SAH). We evaluated brain MRI in a mouse FPM. A total of 28 male C57Bl/6J mice were used. Seventeen animals underwent SAH induction by FPM. In two animals, transient middle cerebral artery occlusion (MCAo) was induced. Nine mice served as controls. T1-weighted images (T1WI), T2-weighted images (T2WI), T2*-weighted images (T2*WI) and apparent diffusion coefficient maps were acquired at day 0 and at various time points following SAH (range: day 1-6 after SAH). Cerebral blood flow (CBF) analysis by C-14-iodoamphetamine (C-14-IMP) autoradiography was conducted in nine animals. Hemorrhage could be best confirmed using T2*WI. The degree of hemorrhage varied. All animals evaluated for >= 2 days were hydrocephalic, which was best seen on T2WI. T2-hyperintensity of the corpus callosum and external capsule, indicating white matter (WM) injury, was present after SAH. Ventricle and WM injury volumes were statistically significantly higher at day 3 compared to day 0. Territorial ischemia was detectable in MCAo but not in SAH. Markedly hypointense cortical veins were visible in the hyperacute and delayed phase after SAH on T2*WI. The C-14-IMP analysis indicated decreased CBF after SAH. MRI is feasible and useful in evaluating pathophysiological changes over time. T2*WI seems best for SAH detection and grading. The chronological change of hydrocephalus and WM injury could be analyzed. T2*WI illustrated specific signal changes of cortical veins, possibly caused by increased oxygen extraction fraction due to decreased CBF. (C) 2016 Elsevier Ltd. All rights reserved.
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