期刊
JOURNAL OF CLINICAL INVESTIGATION
卷 126, 期 6, 页码 2109-2122出版社
AMER SOC CLINICAL INVESTIGATION INC
DOI: 10.1172/JCI84978
关键词
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资金
- Max Planck Society
- European Union [HEALTH-F4-2011-280873]
- EU [643381]
- Fondation pour la Recherche Medicale [DEQ20130326471]
- National Health and Medical Research Council of Australia through a CJ Martin Biomedical Early Career Fellowship grant [APP1052764]
IFN-gamma is a critical mediator of host defense against Mycobacterium tuberculosis (Mtb) infection. Antigen-specific CD4(+) T cells have long been regarded as the main producer of IFN-gamma in tuberculosis (TB), and CD4+ T cell immunity is the main target of current TB vaccine candidates. However, given the recent failures of such a TB vaccine candidate in clinical trials, strategies to harness CD4-independent mechanisms of protection should be included in future vaccine design. Here, we have reported that noncognate IFN-gamma production by Mtb antigen-independent memory CD8(+) T cells and NK cells is protective during Mtb infection and evaluated the mechanistic regulation of IFN-gamma production by these cells in vivo. Transfer of arenavirus- or protein-specific CD8(+) T cells or NK cells reduced the mortality and morbidity rates of mice highly susceptible to TB in an IFN-gamma-dependent manner. Secretion of IFN-gamma by these cell populations required IL-18, sensing of mycobacterial viability, Mtb protein 6-kDa early secretory antigenic target-mediated (ESAT-6-mediated) cytosolic contact, and activation of NLR family pyrin domain-containing protein 3 (NLRP3) inflammasomes in CD11c(+) cell subsets. Neutralization of IL-18 abrogated protection in susceptible recipient mice that had received noncognate cells. Moreover, improved Mycobacterium bovis bacillus Calmette-Guerin (BCG) vaccine-induced protection was lost in the absence of ESAT-6-dependent cytosolic contact. Our findings provide a comprehensive mechanistic framework for antigen-independent IFN-gamma secretion in response to Mtb with critical implications for future intervention strategies against TB.
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