期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 1029, 期 -, 页码 205-212出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2016.07.013
关键词
Malondialdehyde; Plasma; 3-Nitrophenylhydrazine; Derivatization; LC/MRM-MS; Isotope-labeling
资金
- Genome British Columbia
- Genome Canada as part of the Genomics Innovation Network (GIN)
- Metabolomics Innovation Centre (TMIC)
Malondialdhyde (MDA) is a commonly used marker of lipid peroxidation in oxidative stress. To provide a sensitive analytical method that is compatible with high throughput, we developed a multiple reaction monitoring-mass spectrometry (MRM-MS) approach using 3-nitrophenylhydrazine chemical derivatization, isotope-labeling, and liquid chromatography (LC) with electrospray ionization (ESI)-tandem mass spectrometry assay to accurately quantify MDA in human plasma. A stable isotope-labeled internal standard was used to compensate for ESI matrix effects. The assay is linear (R-2=0.9999) over a 20,000-fold concentration range with a lower limit of quantitation of 30 fmol (on-column). Intra- and inter-run coefficients of variation (CVs) were <2% and similar to 10% respectively. The derivative was stable for >36 h at 5 degrees C. Standards spiked into plasma had recoveries of 92-98%. When compared to a common LC-UV method, the LC-MS method found near-identical MDA concentrations. A pilot project to quantify MDA in patient plasma samples (n = 26) in a study of major depressive disorder with winter-type seasonal pattern (MDD-s) confirmed known associations between MDA concentrations and obesity (p < 0.02). The LC-MS method provides high sensitivity and high reproducibility for quantifying MDA in human plasma. The simple sample preparation and rapid analysis time (5x faster than LC-UV) offers high throughput for large-scale clinical applications. (C) 2016 Elsevier B.V. All rights reserved.
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