期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 1021, 期 -, 页码 221-228出版社
ELSEVIER
DOI: 10.1016/j.jchromb.2015.08.001
关键词
Magnetic molecularly imprinted polymers; Surface molecularly imprinted polymers; Roxithromycin; Human plasma; Liquid chromatography-tandem mass spectrometry
资金
- National Natural Science Fund of China [51078108]
- Development Program of China (863 Program) [2011AA060905]
- Program for New Century Excellent Talents in University [NCET11-0795]
In this paper, a simple and reproducible method for the determination of roxithromycin in human plasma samples is proposed. The surface magnetic molecularly imprinted polymers (MMIPs) were utilized as sorbent. Roxithromycin was used as imprinted compound. The experimental results showed that the MMIPs had high affinity and selectivity toward roxithromycin. The extraction process was carried out in a single step by mixing the extraction solvent, MMIP5 and human plasma samples by vortex. When the extraction process was completed, the MMIPs adsorbed the analyte were separated from the sample matrix by an external magnet due to the magnetism. The analyte eluted from the MMIPs was analysed by liquid chromatography-tandem mass spectrometry. Some main factors affecting the extraction of roxithromycin such as the amount of MMIPs, extraction solvent, extraction time, washing and elution conditions were optimized in this study. The calibration curve obtained by analyzing matrix-matched standards showed excellent linear relationship (r(2) = 0.9997) in the concentration range of 10-1000 ng mL(-1). The limit of detection and quantification obtained were 3.8 and 9.8 ng mL(-1), respectively. The relative standard deviations of intra- and inter-day obtained were in the range of 3.9 %-5.5 % and 2.9 %-4.6 % with the recoveries ranging from 86.5 % to 91.5 %. (C) 2015 Elsevier B.V. All rights reserved.
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