期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 1033, 期 -, 页码 317-320出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2016.08.046
关键词
7 alpha-Hydroxy-4-cholesten-3-one; Bile acids; Cholesterol 7 alpha-hydroxylase; Mass spectrometry
资金
- Czech Ministry of Health [RVO-VFN64165]
The serum concentration of 7 alpha-hydroxy-4-cholesten-3-one (C4), a marker of cholesterol 7 alpha-hydroxylase activity, has recently become an attractive diagnostic tool for researchers interested in cholesterol and bile acid metabolism. The rapidly increasing demand of C4 measurement led to the development of various fast, mostly mass spectrometry-based analytical methods. Our aim was to compare four simple (i.e., not requiring solid phase extraction) extraction procedures (two one-phase, and two two-phase) in terms of basic analytical performance and their labouriousness. All methods exhibited comparable extraction recoveries (ranging from 88 to 97%) and intra-assay precision (variation coefficients below 10%), and failed in the removal of phospholipids. Although marked differences were observed in desalting and deproteination, all methods can be considered satisfactory. Simple acetonitrile precipitation can be recommended if a fast extraction and minimal hands-on time is preferred; while two-phase ammonium sulphate:acetonitrile extraction should be chosen when maximal deproteination is required. (C) 2016 Elsevier B.V. All rights reserved.
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