期刊
JOURNAL OF ANALYSIS AND TESTING
卷 6, 期 1, 页码 44-52出版社
SPRINGER SINGAPORE PTE LTD
DOI: 10.1007/s41664-022-00212-4
关键词
CRISPR-Cas12; Nucleic acid detection; Sensitivity; Integrated detection; Simplified detection mode; Quantitative detection
资金
- National Natural Science Foundation of China [91959128, 21874049]
This review paper discusses recent advances in CRISPR-Cas12-based nucleic acid detection, with a focus on the methods and means to improve the detection capability of CRISPR-Cas12. Strategies for enhancing sensitivity, optimizing integrated detection, developing simplified detection mode, and improving quantitative detection capabilities are summarized, along with a prospect of future developments in CRISPR-Cas12-based nucleic acid detection.
The COVID-19 pandemic has brought great challenges to traditional nucleic acid detection technology. Thus, it is urgent to develop a more simple and efficient nucleic acid detection technology. CRISPR-Cas12 has signal amplification ability, high sensitivity and high nucleic acid recognition specificity, so it is considered as a nucleic acid detection tool with broad development prospects and high application value. This review paper discusses recent advances in CRISPR-Cas12-based nucleic acid detection, with an emphasis on the new research methods and means to improve the nucleic acid detection capability of CRISPR-Cas12. Strategies for improving sensitivity, optimization of integrated detection, development of simplified detection mode and improvement of quantitative detection capabilities are included. Finally, the future development of CRISPR-Cas12-based nucleic acids detection is prospected.
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