期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 1029, 期 -, 页码 213-221出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2016.06.024
关键词
LC-MS/MS; Simultaneous assessment; Thiol compounds
资金
- National Natural Science Foundation of China [81373471, 81430086]
- program for Innovative Research Team of the Ministry of Education
- Program for Liaoning Innovative Research Team in University
Biological thiol compounds are very important molecules that participate in various physiological events. Alteration in levels of endogenous thiols has been suggested as a biomarker of early stage of pathological changes. We reported a chemical derivatization- and LC-MS/MS-based approach to simultaneously determine thiol compounds including glutathione (GSH), cysteine (Cys), N-acetyl cysteine (NAC), homocysteine (Hcy), and cysteinylglycine (CysGly) in biological samples. Thiol-containing samples were derivatized with monobromobimane (mBrB) at room temperature, followed by LC-MS/MS analysis. Assessment of the analytes with baseline separation was completed within 10 min, using a gradient elution on a C-18 reversed-phase column. Excellent linearity was observed for all analytes over their concentration ranges of 1-400 mu M. The lowest limits of detection (S/N = 3) in a range from 0.31 fmol (for NAC) to 4.98 fmol (for CysGly) were achieved. The results indicate that this approach was sensitive, selective, and well suited for high-throughput quantitative determination of the biological thiols. (C) 2016 Elsevier B.V. All rights reserved.
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