期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1462, 期 -, 页码 165-169出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2016.08.002
关键词
UHPLC; Packed capillary columns; High slurry concentrations; Sonication; High efficiency
资金
- Waters Corporation (Milford, MA)
- National Institute of Health [5U24DK097153]
- National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) [1R01DK101473-01A1]
Slurry packing capillary columns for ultrahigh pressure liquid chromatography is complicated by many interdependent experimental variables. Previous results have suggested that combination of high slurry concentration and sonication during packing would create homogeneous bed microstructures and yield highly efficient capillary columns. Herein, the effect of sonication while packing very high slurry concentrations is presented. A series of six, 1 m x 75 mu m internal diameter columns were packed with 200 mg/mL slurries of 2.02 mu m bridged-ethyl hybrid silica particles. Three of the columns underwent sonication during packing and yielded highly efficient separations with reduced plate heights as low as 1.05. (C) 2016 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据