期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1436, 期 -, 页码 141-146出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2016.01.070
关键词
Early synthetic dyes; Art conservation; Comprehensive two-dimensional liquid chromatography; LC x LC; Fast ion-pair LC
资金
- Netherlands Organisation for Scientific Research (NWO) [C.2322.0291]
- Agilent Technologies
In the late 19th century, newly invented synthetic dyes rapidly replaced the natural dyes on the market. The characterization of mixtures of these so-called early synthetic dyes is complicated through the occurrence of many impurities and degradation products. Conventional one-dimensional liquid chromatography does not suffice to obtain fingerprints with sufficient resolution and baseline integrity. Comprehensive two-dimensional liquid chromatography (LC x LC) is employed in this study, with ion exchange chromatography in the first dimension and fast ion-pair liquid chromatography in the second. Retention in the first dimension is largely determined by the number of charges, while the selection of a small ion-pair reagent (tetramethylammonium hydroxide) in the second dimension causes retention to be largely determined by the molecular structure of the dye. As a result, there is a high degree of orthogonality of the two dimensions, similar to the values typically encountered in GC x GC. The proposed LC x LC method shows a theroretical peak capacity of about 2000 in an analysis time of about three hours. Clear, informative fingerprints are obtained that open a way to a more efficient characterization of dyes used in objects of cultural heritage. (C) 2016 Elsevier B.V. All rights reserved.
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