期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1457, 期 -, 页码 66-75出版社
ELSEVIER
DOI: 10.1016/j.chroma.2016.06.031
关键词
Antibody aggregate; Protein A adsorption; Higher order structure; HDX-MS; Protein Conformational Array; Molecular modelling
资金
- Process Development Team in Bristol-Myers Squibb Company at the Devens site
- Bristol-Myers Squibb Company
- Process Analytical Team in Bristol-Myers Squibb Company at the Devens site
Antibody aggregate is a common issue in therapeutic antibodies, which may compromise product efficacy and cause adverse effects. Antibody aggregate level is normally controlled in bioprocessing by polishing steps after Protein A capture. This paper studied the Higher Order Structures (HOS) of antibody aggregates (dimer H1 and H2) and their adsorption on Protein A resin and thus elucidated the mechanism using Protein A capture for enhanced aggregate removal. The HOS of antibody aggregates and their complex with Protein A were characterized using HDX-MS combined with SEC-MALS, Protein Conformational Array (PCA), and molecular modeling. The aggregate size and Protein A binding ratio suggested that H2 has much more compact structure than H1. HDX-MS and PCA further revealed that H1 was formed by single Fab-Fab interaction while H2 formed by Fab-Fab and likely Fc-Fc interaction. On Protein A resin, both the molar binding ratio and the correlation between protein size and ligand distance support that each monomer can only bind one Protein A ligand, while each dimer can bind two ligands, thus resulting in stronger resin binding. Furthermore, dimer H2 binds stronger than dimer H1 due to its compact structure. By integrating biophysical analysis and molecular modeling with process development, this study revealed the antibody aggregate structures and the mechanism of aggregate removal using Protein A chromatography. It also provided a general strategy for in-depth product and process understanding in antibody and other biologics development. (C) 2016 Elsevier B.V. All rights reserved.
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