期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1449, 期 -, 页码 8-16出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2016.04.044
关键词
Ball mill assisted mechanochemical extraction; Ionic liquid; Natural product from plant; Tanshinones; Sample preparation
资金
- Program of Natural Science Research of Jiangsu Higher Education Institutions of China [13KJB150025]
- National Natural Science Foundation of China [21447003, 21507062]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- Jiangsu Collaborative Innovation Center of Biomedical Functional Materials
- Chinese National Scholarship for Graduate Students
A ball mill assisted mechanochemical extraction method was developed to extract compounds of natural product (NP) from plant using ionic liquid (IL). A small volume ball mill, also known as PastPrep (R) Homogenizer, which is often used for high-speed lysis of biological samples and for other applications, was used to dramatically increase the speed, completeness and reproducibility of the extraction process at room temperature to preserve the chemical integrity of the extracted compounds. In this study, tanshinones were selected as target compounds to evaluate the performance of this extraction method. Factors affecting the extraction efficiency, such as the duration, IL concentration and solid/liquid ratio were systematically optimized using the response surface methodology. Under the optimized conditions, the described method was more efficient and much faster than the conventional extraction methods such as methanol based ultrasound assisted extraction (UAE) and heat reflux extraction (HRE) that consumes a lot more organic solvent. In addition, the natural products of interest were enriched by anion metathesis of ionic liquids, combining extraction and preconcentration in the same process. The extractant was analyzed by HPLC and LC-MS. The reproducibility (RSD, n=5), correlation coefficient (r(2)) of the calibration curve, and the limit of detection, were determined to be in the range of 4.7-5.2%, 0.9992-0.9995, and 20-51 ng/mL, respectively. (C) 2016 Elsevier B.V. All rights reserved.
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