期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1444, 期 -, 页码 99-105出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2016.03.071
关键词
Countercurrent chromatography; Cyclodextrins; Selective isolation; Separation mechanism; Volatile oil
资金
- Department of Education of Zhejiang Province of China [pd2013031]
- Natural Science Foundation of China [81503219]
Selective separation of chemical components from seven kinds of volatile oil by countercurrent chromatography with three types of cyclodextrins as selective reagent was investigated in this work. Preparative separation of chemical components from volatile oil is generally quite challenging due to their extremely complexity of the composition. A biphasic solvent system n-hexane-0.10 mol L-1 cyclodextrin (1:1, v/v) was selected for separation of components from volatile oil and three types of cyclodextrins were investigated, including beta-cyclodexrin, methyl-beta-cyclodexrin and hydroxypropy1-beta-cyclodexrin. All kinds of volatile oils are from seven kinds of traditional Chinese herb. Results showed that some chemical components could be well separated with high purity from each kind of volatile oil using different type of cyclodextrin as selective reagent. For example, germacrone and curcumenone could be selectively separated from volatile oil of Curcumae Rhizoma with methyl-beta-cyclodexrin and hydroxypropyl-beta-cyclodexrin as selector respectively, and other five components were selectively separated from volatile oil of Chuanxiong Rhizoma, Myristicae Semen, Aucklandiae Radix and Angelicae Sinensis Radix by countercurrent chromatography with different cyclodexrin as selective reagent. Separation mechanism for separation of components from volatile oil by countercurrent chromatography with cyclodextrin as selective reagent was proposed. Peak resolution of the present separation method could be greatly influenced by the chemical compositions of volatile oil. (C) 2016 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据