期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1462, 期 -, 页码 44-54出版社
ELSEVIER
DOI: 10.1016/j.chroma.2016.07.069
关键词
Arsenic speciation; Wines; Ionic liquids; Liquid chromatography; Atomic fluorescence spectrometry
资金
- Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET)
- Agencia Nacional de Promocion Cientifica y Tecnologica (FONCYT) (PICT-BID)
- Organization for the Prohibition of Chemical Weapons (OPCW)
The application of different ionic liquids (ILs) as modifiers for chromatographic separation and determination of arsenite [As(III)], arsenate [As(V)], dimethylarsonic acid (DMA) and monomethylarsonic acid (MMA) species in wine samples, by reversed-phase high performance liquid chromatography coupled to hydride generation atomic fluorescence spectrometry detection (RP-HPLC-HG-AFS) was studied in this work. Several factors influencing the chromatographic separation of the As species, such as pH of the mobile phase, buffer solution concentration, buffer type, IL concentration and length of alkyl groups in ILs were evaluated. The complete separation of As species was achieved using a C-18 column in isocratic mode with a mobile phase composed of 0.5% (v/v) 1-octyl-3-methylimidazolium chloride ([C-8 mim]Cl) and 5% (v/v) methanol at pH 8.5. A multivariate methodology was used to optimize the variables involved in AFS detection of As species after they were separated by HPLC. The ILs showed remarkable performance for the separation of As species, which was obtained within 18 min with a resolution higher than 0.83. The limits of detection for As(III), As(V), MMA and DMA were 0.81, 0.89, 0.62 and 1.00 mu g As L-1. The proposed method was applied for As speciation analysis in white and red wine samples originated from different grape varieties. (C) 2016 Elsevier B.V. All rights reserved.
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