期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1432, 期 -, 页码 39-48出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2015.12.083
关键词
Phosphodiester stationary phase; Phospholipids; Fatty acids; HPLC; MALDI-TOF MS
资金
- National Science Center (Cracow, Poland) [NCN 2013/11/N/ST4/01838, NCN 2013/09/D/ST4/03807]
- budget of Kuyavia and Pomerania
- European Regional Development Fund under the ROP [RPKP.05.04.00-04-003/13]
- Krok w przyszlosc (Step into the Future) Sectoral Operational Program Human Resources
A methodology employing high performance liquid chromatography coupled with matrix-assisted laser desorption and ionization time-of-flight mass spectrometry has been utilized to determine the quality of phospholipid classes. Home-made phosphoester chemically bonded stationary phase containing diol, phosphate and octadecyl groups (Diol-P-C18) has been employed in the separation of polar lipids from milk. Each phospholipid fraction was collected manually and identified by MALDI-TOF MS. (C) 2015 Elsevier B.V. All rights reserved.
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