期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1438, 期 -, 页码 179-188出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2016.02.032
关键词
Hydrophilic interaction liquid chromatography; Retention; Oligosaccharides; Liquid chromatography/mass spectrometry; Fluorescent derivatives
资金
- Czech Science Foundation [14-06319S]
- Czech Academy of Sciences [RVO: 68081715]
In this work, we have investigated retention of maltooligosaccharides and their fluorescent derivatives in hydrophilic interaction liquid chromatography using four different stationary phases. The non-derivatized maltooligosaccharides (maltose to maltoheptaose) and their derivatives with 2-aminobenzoic acid, 2-aminobenzamide, 2-aminopyridine and 8-aminonaphthalene-1,3,6-trisulfonic acid were analyzed on silica gel, aminopropyl silica, amide (carbamoyl-bonded silica) and ZIC-HILIC zwitterionic sulfobetain bonded phase. The partitioning of the analytes between the bulk mobile phase and adsorbed water-rich layer, polar and ionic interactions of analytes with stationary phase have been evaluated and compared. The effects of the mobile phase additives (0.1% (v/v) of acetic acid and ammonium acetate in concentration range 5-30 mmol L-1) on retention were described. The suitability of different models for prediction of retention was tested including linear solvent strength model, quadratic model, mixed-mode model, and empirical Neue-Kuss model. The mixed-mode model was extended to the parameter describing the contribution of monomeric glucose unit to the retention of non-derivatized and derivatized maltooligosaccharides, which was used for evaluation of contribution of both, oligosaccharide backbone and end-group to retention. (C) 2016 Elsevier B.V. All rights reserved.
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