期刊
JOURNAL OF CHROMATOGRAPHIC SCIENCE
卷 54, 期 8, 页码 1271-1276出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/chromsci/bmw082
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The stimulating herbal drug khat (cathine, cathinone) and its analog methcathinone are common substances of abuse in most countries. A GC-MS method was developed and validated for the detection and quantification of cathine, cathinone, methcathinone and ephedrine in oral fluid specimens. The analytes and internal standard (amphetamine-d5) were extracted from 0.5 mL oral fluids by ethyl acetate, and then the dried extracts were derivatized with heptafluorobutyric anhydride at 70 degrees C for 30 min. The MS was used in selected ion monitoring mode. Ions monitored were m/z 117, 240 and 330 for cathine, m/z 77, 105 and 240 for cathinone, m/z 105, 210 and 254 for methcathinone, m/z 210, 254 and 344 for ephedrine andm/z 244 and 336 for amphetamine-d(5). The calibration curves were linear (r(2) > 0.98) in the concentration range 20-2,000 ng/mL for all analytes. The intra-and inter-assay imprecisions were within (1.6-12.5%) and (1.5-9.5%), respectively, for all analytes. Intra-assay accuracies were between -5.9 and 6.7% for all analytes. The method was successfully applied to detect and quantify the target analytes from oral fluid specimens collected from Khat and methcathinone users.
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