期刊
JOURNAL OF CHEMICAL NEUROANATOMY
卷 74, 期 -, 页码 21-27出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchemneu.2016.02.002
关键词
Claustrum; Cannabinoid; CB1R; Dog; FAAH; Immunohistochemistry
资金
- Innovet Italia Srl (Milan, Italy)
Cannabinoid receptor 1 (CB1R) and fatty acid amide hydrolase (FAAH) are part of the endocannabinoid system (ECB) which exerts a neuromodulatory activity on different brain functions and plays a key role in neurogenesis. Although many studies have reported FAAH and CB1R expression in the brain of different animal species, to the best of our knowledge they have never been described in the canine claustrum. Claustrum samples, obtained from necropsy of four neurologically normal dogs, were formalin fixed for paraffin embedding. Sections were either stained for morpho-histological analysis or immunostained for CB1R and FAAH. Analysis of adjacent sections incubated with the two antisera showed a complementary labeling pattern in the claustrum, with CB1R antibody staining fibers while anti-FAAH antibody stained cell bodies and the proximal portion of dendrites; this particular anatomical relationship suggests a retrograde endocannabinoid action via CB1R. CB1R and FAAH complementary immunostaining and their cellular localization reported here provide the first anatomical evidence for existence of the ECB in the dog claustrum. (C) 2016 Elsevier B.V. All rights reserved.
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