期刊
DATA IN BRIEF
卷 42, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.dib.2022.108055
关键词
Mass-spectrometry; Proteomics; Shotgun; SRM SIS; Fractionation
资金
- Russian Science Foundation, RSF [20-15-00410]
- Russian Science Foundation [20-15-00410] Funding Source: Russian Science Foundation
The data was obtained from 3 normal human liver tissues using LC-MS methods. Different LC-MS approaches were employed to analyze the samples and obtain qualitative and semi-quantitative information about the human liver proteome, as well as absolute quantitative concentrations of proteins encoded by human chromosome 18.
The data was acquired from 3 normal human liver tissues by LC-MS methods. The tissue liver samples from male subjects post mortem were obtained from ILSBio LLC (https://bioivt.com/). Liver tissue was frozen in liquid nitrogen, transported and shipped on dry ice. The proteins were extracted and purified followed up by trypsin hydrolysis. The peptide mixture was aliquoted and analyzed by different LC-MS approaches: one-dimensional shotgun LC-MS, two-dimensional LC-MS, two-dimensional SRM SIS (Selected Reaction Monitoring with Stable Isotope-labeled peptide Standards). The Shotgun assay resulted in a qualitative in-depth human liver proteome, and a semi-quantitative iBAQ (intensity-based absolute quantification) value was calculated to show the relative protein content of the sample. Absolute quantitative concentrations of proteins encoded by human chromosome 18 using SRM SIS were obtained. (c) 2022 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)
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