A mice resting-state functional magnetic resonance imaging dataset on the effects of medetomidine dosages and prior-stimulation on functional connectivity

Nine 8 C57Bl6 mice (9 +/- 0.5 months) were utilised for this dataset. Each animal was scanned twice on a 9.4T Bruker Magnetic Resonance Imaging (MRI) scanner using a cryogenically cooled coil with 0.1 mg/kg body weight/h (low) or 0.3 mg/kg body weight/h (high) medetomidine doses; 0.5% isoflurane was used in conjunction with both doses. The scans were one week apart, and the first session's dose was decided randomly. In each session, the animal had a pre-stimulation resting-state functional Magnetic Resonance Imaging (rs-fMRI) scan followed by 10 min where mild, constant electrical stimulation to the forepaw was applied, and a post-stimulation rs-fMRI scan. Each fMRI scan lasted 10 min, and there was 5 min break between fMRI scans. The dataset included, for each animal, a pair of forwardphase and reverse-phase gradient echo Echo-Planar-Imaging (EPI) images for EPI distortion correction purpose and three (unprocessed) functional MRI images acquired using the same EPI sequence: prior, during, and post-stimulation. The MRI data was saved in compressed NIFTI format converted from Bruker DICOMs. The dataset also included the preprocessed functional MRI images, with the following preprocessing steps: slice-timing correction, temporal despiking, motion correction, distortion correction, band-pass filtration at 0.01-0.2 Hz, and spatial normalisation. This dataset adds to the publicly available collection of resting-state functional MRI in the mice and facilitates reproducibility and validation of functional imaging and its analysis. (C) 2022 The Authors. Published by Elsevier Inc.

Automated summary

This study utilized nine 8C57Bl6 mice for MRI scanning, involving drug injections at different doses and electrical stimulation. The dataset includes preprocessed fMRI images and various correction steps.