期刊
JOURNAL OF CEREAL SCIENCE
卷 69, 期 -, 页码 85-94出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jcs.2016.02.014
关键词
Wheat grain; Nitrogen fertilizer; Metabolome; Flour quality; Metabolite-metabolite correlations
资金
- National Natural Science Foundation of China [31471485]
- Natural Science Foundation of Beijing City
- Key Developmental Project of Science and Technology from Beijing Municipal Commission of Education [KZ201410028031]
- International Science & Technology Cooperation Program of China [2013DFG30530]
Wheat yield and quality are dependent largely on nitrogen (N) availability. In this study, we performed the first metabolomic analysis of the response to high-N fertilizer during wheat grain development using non-targeted gas chromatography-mass spectrometry (GC MS). Quality parameter analyses demonstrated that high-N fertilizer application led to a significant increase in grain protein content and improvement in starch and bread-making quality. Comparative metabolomic profiling of six grain developmental stages resulted in identification of 74 metabolites, including amino acids, carbohydrates, organic acids and lipids/alcohol, which are primarily involved in carbon and N metabolism. Under high-N fertilizer treatment, numerous metabolites accumulated significantly during grain development. Principal component analysis revealed two principal components as being responsible for the variances resulting from N-fertilizer treatments. Metabolite metabolite correlation analysis demonstrated that the high-N treatment group had a greater number of positive correlations among metabolites, suggesting that high-N fertilizer treatment induced a concerted metabolic change that resulted in improved grain development. Particularly, the high-N treatment-mediated significant accumulation of metabolites involved in the TCA cycle, starch and storage protein synthesis could be responsible for the improvement of grain yield and quality. Our results provide new insight into the molecular mechanisms of wheat grain development and yield and quality. (C) 2016 Elsevier Ltd. All rights reserved.
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