Study on hypoglycemic effects of irradiated ginseng adventitious roots

We aimed to explore the effects of the Co-60-gamma irradiated ginseng adventitious root (GAR) with different radiation doses on the hypoglycemic effects of its extract (GARSE) through in vivo and in vitro experiments. The total saponin of GARSE was increased by 4.50% after 5 kGy irradiation, and the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability was enhanced by 5.10%. At 50 mu g/mL, GARSE irradiated by 5 kGy displayed superior protective effects on human glomerular mesangial cells (HMCs) with high glucose damage. After feeding type 1 diabetes mellitus (T1DM) mice with GARSE irradiated by 5 kGy at 500 mg/kg center dot BW for 4 weeks, the glucose values was decreased by 16.0% compared with the unirradiated. The Keap1/Nrf2/HO-1 pathway was activated and the oxidative stress was attenuated, which further alleviated T1DM.

Automated summary

This study explored the effects of different radiation doses on the hypoglycemic effects of the Co-60-gamma irradiated ginseng adventitious root extract. The irradiation increased the saponin content and radical scavenging ability of the extract. The irradiated extract showed superior protection on glomerular mesangial cells and significantly reduced glucose levels in mice with type 1 diabetes, possibly through the activation of the Keap1/Nrf2/HO-1 pathway and attenuation of oxidative stress.