期刊
FOOD CHEMISTRY-X
卷 14, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.fochx.2022.100308
关键词
Ochratoxin A; Malt; Fluorescent aptasensor; Graphene oxide nanosheet; Double-stranded DNA
资金
- Beijing Natural Science Foundation [7222285]
- Open Project of Key Laboratory of Modern Preparation of TCM, Ministry of Education, Jiangxi University of Chinese Medicine [zdsys-202109]
- S&T Program of Hebei [21344801D]
A fluorescent aptasensor based on graphene oxide nanosheet and double-stranded DNA structure was developed for OTA detection. The sensor exhibited outstanding sensitivity and selectivity, making it a promising tool for food safety applications.
A signal off fluorescent aptasensor based on graphene oxide (GO) nanosheet and double-stranded DNA structure was fabricated for OTA detection. In the absence of OTA, the aptamer and its complementary DNA (cDNA) formed double-stranded conjugates that could coexist with GO, presenting fluorescence responses. Then, the presented OTA was captured by the aptamers, causing the release of cDNA-FAM probes. The free probes were adsorbed by GO, leading to an OTA concentration-dependent fluorescence quenching through fluorescence resonance energy transfer. Under optimum conditions, the fluorescent aptasensor exhibited outstanding sensitivity with a LOD of 11 pg/mL and a wide dynamic range of 0.04-30 ng/mL. The selectivity of the aptasensor was confirmed against other four mycotoxins, and the feasibility and reliability were verified by determining OTA in the spiked malt samples with satisfactory recovery of 95.50%-112.05%. This aptasensing platform could be adapted to measure other mycotoxins by replacing the aptamer sequences for food safety.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据