期刊
FOOD CHEMISTRY-X
卷 14, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.fochx.2022.100262
关键词
Citrus peels; Ultra-performance liquid chromatography; coupled to photodiode array detection; Ultra-high performance liquid; chromatography-quadrupole time-of-flight; mass spectrometry; Fused-core column; Comprehensive analysis
资金
- Sao Paulo Research Foundation (FAPESP) [2018/14582-5, 2019/13496-0]
- FAPESP [2020/15774-5, 2020/08421-9]
- Coordenacao de Aperfei-coamento de Pessoal de Nivel Superior-Brazil (CAPES) [88887.310558/2018-00, 001]
In this study, a method based on ultra-high-performance liquid chromatography with a photodiode array detector (UPLC-PDA) was developed for comprehensive analysis of phenolic compounds in citrus peels. The method was successfully applied to various citrus peel species, detecting multiple phenolic compounds and limonoids, and verified for accuracy and precision.
In this work, a method based on ultra-high-performance liquid chromatography with a photodiode array detector (UPLC-PDA) was developed to comprehensively analyze phenolic compounds in peels of lime (Citrus x latifolia), lemon (Citrus limon), and rangpur lime (Citrus x limonia). The reverse-phase separation was achieved with a C18 fused-core column packed with the smallest particles commercially available (1.3 um). The method was successfully coupled with high-resolution mass spectrometry (HRMS), allowing the detection of 24 phenolic compounds and five limonoids in several other citrus peels species: key lime, orange and sweet orange, tangerine, and tangerine ponkan, proving the suitability for comprehensive analysis in citrus peel matrices. Additionally, the developed method was validated according to the Food and drug administration (FDA) and National Institute of Metrology Quality and Technology (INMETRO) criteria, demonstrating specificity, linearity, accuracy, and precision according to these guidelines. System suitability parameters such as resolution, tailoring, plate count were also verified.
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