4.7 Article

Microporosity engineered printable silk/graphene hydrogels and their cytocompatibility evaluations

期刊

MATERIALS TODAY ADVANCES
卷 14, 期 -, 页码 -

出版社

ELSEVIER
DOI: 10.1016/j.mtadv.2022.100233

关键词

Silk fibroin; Reduced graphene oxide; Hybrid inks; Hydrogel; Rheo-SANS; Extrusion printing; Tissue engineering

资金

  1. Australian Research Council (ARC) [DP160101267]
  2. ARC Industry Transformation Research Hub [IH150100003, P7428]

向作者/读者索取更多资源

In this study, a regenerated silk fibroin/reduced graphene oxide hybrid ink system was developed and used to fabricate hydrogels with tunable properties and cell-gel interactions through extrusion printing and photocrosslinking. The incorporation of isopropyl alcohol (IPA) and increase in rGO content affected the viscosity, contact angle, printing accuracy, and physical properties of the hybrid ink and hydrogels. The increase in rGO content also improved the crosslink density, mechanical properties, and decreased the micropore size of the hybrid hydrogels. Furthermore, the hybrid and bioprinted hydrogels showed good biocompatibility with marginal difference in cell viability. These findings suggest the potential application of the developed hydrogel systems in tissue engineering and other functional applications.
In this work, regenerated silk fibroin (RSF)/reduced graphene oxide (rGO) hybrid ink system was fabricated in aqueous phase, extrusion printed and photocrosslinked to hydrogels with tunable physicochemical properties and cell-gel interactions. Aqueous isopropyl alcohol was used to disperse the rGO into RSF matrix, where the RSF molecules showed relatively increased radius of gyration with IPA resulting in increased pore size and water uptake capacity of the photocrosslinked hydrogels. Incorporation of IPA along with increase in rGO content systematically decreased the viscosity, contact angle and printing accuracy of RSF/rGO hybrid inks, where the ink with highest rGO content exhibited relatively loose network structure at printing shear rate. However, no secondary structural change and fibrillo-genesis was observed under printing shear stress, making it a suitable system for bioprinting silk hydrogel scaffolds. In addition, increase in rGO content systematically increased the crosslink density, beta-sheet content, and mechanical properties of RSF/rGO hybrid hydrogels, whereas decreased the micropore size and water uptake capacity. Moreover, with increase in rGO content, the hybrid and bioprinted hydrogels showed good biocompatibility with marginal difference in cell viability on hybrid and bioprinted gels. The developed hydrogel systems could be potentially applied for tissue engineering and other functional applications. (C) 2022 Published by Elsevier Ltd.

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